Vaccinium uliginosum L. (VU) possesses various biological properties, such as antioxidant and protective effects against VUinduced\r\nskin photoaging. The purpose of this study is to evaluate the effects of oral administration of a mixture of polyphenols\r\nand anthocyanins derived from VU on 2,4-dinitrochlorobenzene- (DNCB-) induced atopic dermatitis (AD) in NC/Nga mice.\r\nWe assessed anti-AD effects in NC/Nga murine model for a period of 9 weeks. Oral administration of the mixture significantly\r\nalleviated the AD-like skin symptoms and clinical signs including ear thickness and scratching behaviors. Orally administrated\r\nmixture reduced the level of IgE and IgG1, whereas it increased the level of IgG2a in a dose-dependent manner. The calculated\r\nIgG1/IgG2a ratio for each mouse revealed that the mixture derived from VU also significantly reduced the Th2/Th1 ratio, IL-4 and\r\nIL-13 (as Th2 cytokines), IFN-?, and IL-12 (as a Th1 cytokine) in spleens. In addition, it significantly decreased gene expression,\r\nsuch as IL-4, IL-5, CCR3, eotaxin-1, IL- 12, IFN-?, MCP-1, and IL-17, in AD-like lesions and suppressed Th17. Histological\r\nanalyses revealed that the epidermis thickness and number of inflammatory cells were significantly reduced. In conclusion, oral\r\nadministration of the mixture in the DNCB-induced AD is confirmed to improve AD disease in mice.
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